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KMID : 1024519960050010043
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Journal of the Environmental Sciences
1996 Volume.5 No. 1 p.43 ~ p.50
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Purification and Characterization of Oxidase Induced from Thiobacillus neapolitanus R-10
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Won Yong-Don
Kim Dong-Suk
Ryu Beung-Ho
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Abstract
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Thiobacillus neopolitanus R-10, which produces a active thiosulfate oxidase was isolated from nightsoil. The optimal culture conditions of Thiobacillus neopolitanus R-10 for the production of enzyme was determined as followed: 0.8% , 0.2% , 0.2% , 0.04% , 0.02% ., 2ml trace elements solution, ann PH 6.5 at 3 and 72hr cultivation. The oxidase was successively purified 83 folds yield by fractionation, DEAE-Cellulose, Sephadex A-50 column chromatogrophy and gel Sephadex G-150 gel filteration with yield of 5.9%. The molecular weight of purified enzyme was estimated to be 43.000 dalton by SDS-polyacrylamide gel electrophoresis and gel filteration column chromatography The enzyme activity was highest at 40t and PH 7.0 The enzyme activity was relatively high by -mercaptoethanol but strongly inhibited by cysteine. Key Words : thiobacillus neapolitanus
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KEYWORD
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Thiobacillus neopolitanus, thiosulfate oxidase
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